Tylka Nematology Lab

Procedure:

• Construct microsieves from 18-mm and 20-mm test tube caps and 25 micrometer pore nylon mesh.
• UV sterilize all microsieves and hatch trays.
• Arrange hatching trays in replicated boxes using pre-generated random order.
• Fill each hatching tray with 12 ml of the appropriate test or control solution.
• Pipette between 5,000 and 10,000 (depending on experimental details) surface disinfested SCN eggs onto each microsieve.
• Incubate the experiment at room temperature in darkness.
• At every-other day intervals, transfer the microsieves to duplicate hatching trays containing fresh solution, and count the hatched second-stage juveniles remaining in the original solution.