Protocols
Periodically people inquire about the procedures we use in the lab. We are always available to answer questions about our techniques, but the following information may provide a good starting point.
Standard Operating Procedures for:
1. Routine soil processing for SCN egg counts
- screening soil
- cyst extraction (by hand)
- cyst extraction (automated)
- grinding open cysts to release eggs
- counting eggs
2. Surface disinfestation of eggs
3. Baermann funnel extraction of nematodes from soil
5. Greenhouse resistance screening
6. Egg purification by sucrose centrifugation
7. Staining Roots for Plant-Parasitic Nematode Visualization
8. Acid fuchsin stain preparation
Updated 03/21/2005 - 12:47pm