Tylka Nematology Lab

Procedure:

• Obtain a well mixed soil sample.
• Fill a bucket with 2 quarts of water (marked on inside of bucket).

• Pour in the soil, break any clumps with your fingers, and mix the soil suspension well for 15 seconds.
• Pour the soil suspension through a #20 over a #60 sieve.

• Rinse the debris caught on the top sieve with water, and then discard its contents. Carefully wash the cysts and accompanying sediments from the #60 sieve into a clean beaker or directly into a 100 ml plastic grinding tube using as little water as possible.