Counting SCN Eggs

Procedure:

  • Increase the volume of liquid in a sample to 100 ml with tap water.
  • Examine the empty counting slide under the microscope to be certain that there are no eggs remaining in the slide from previous counts.
  • Thoroughly mix the sample.
  • Immediately after mixing the sample, draw sufficient volume into a glass pasteur pipet to fill the counting slide.
  • Fill the counting slide from the pipet.
    Nematode Counting Slide
  • Systematically move from cell to cell in the slide grid, counting all SCN eggs.
    SCN egg - high power
  • Record total count and dilution.
  • Pour the liquid from the slide back into the sample, and rinse the slide thoroughly with a water bottle.

Sources:

  • Nematode counting slides: Chalex Corporation, 5004 228th Avenue S.E., Issaquah, Washington 98029, (425) 391-1169.

Updated 05/01/2006 - 9:33am