Abstract
Marett, C.C., G.L. Tylka, and K.R. Harkins. 1996. Relationship between embryogeny and hatching of soybean cyst nematode eggs. Phytopathology 86 (supplement):98.
Experiments were conducted to determine whether variation in hatch of soybean cyst nematode eggs incubated in various solutions was due to differences in embryogeny of the unhatched eggs. Eggs were collected from adult females, surface disinfested, then incubated in the dark at 26°C in 100 ppm solutions of two synthetic hatch inhibitors, in a known hatch stimulator (3.0 mM zinc sulfate), or in deionized water (pH 7.0). Every other day, eggs were transferred to fresh solutions and hatched juveniles were counted. After 28 days, total cumulative percent hatch was determined, and unhatched eggs from each treatment were analyzed by flow cytometry to determine embryo development. Differences (P < 0.05) in final percent hatch among treatments were detected; average final cumulative hatch was 40.6% in zinc sulfate, 22.5% in deionized water, and less than 1.5% in the hatch inhibitors. However, no differences in embryogeny of unhatched eggs were detected; more than 85% of the unhatched eggs in all solutions contained vermiform juveniles. The hatch-inhibitory compounds studied apparently had no effect on development of the embryos.