Soybean Cyst Nematode

Periodically people inquire about the procedures we use in the lab. We are always available to answer questions about our techniques, but the following information may provide a good starting point.

Standard Operating Procedures for:

1. Routine soil processing for SCN egg counts

• screening soil
  
• cyst extraction (by hand)
• cyst extraction (automated)
• grinding open cysts to release eggs
• counting eggs

2. Surface disinfestation of eggs

3. Baermann funnel extraction of nematodes from soil

4. In-vitro hatch experiments

5. Greenhouse resistance screening

6. Egg purification by sucrose centrifugation

7. Staining roots for plant-parasitic nematode visualization

8. Acid fuchsin stain preparation