Counting SCN Eggs
Procedure: - Increase the volume of liquid in a sample to 100 ml with tap water.
- Examine the empty counting slide under the microscope to be certain that there are no eggs remaining in the slide from previous counts.
- Thoroughly mix the sample.
- Immediately after mixing the sample, draw sufficient volume into a glass pasteur pipet to fill the counting slide.
- Fill the counting slide from the pipet.
- Systematically move from cell to cell in the slide grid, counting all SCN eggs.
- Record total count and dilution.
- Pour the liquid from the slide back into the sample, and rinse the slide thoroughly with a wash bottle.
Sources: - Nematode counting slides: Chalex Corporation, 5004 228th Avenue S.E., Issaquah, Washington 98029, (425) 391-1169.
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