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Qualitative and comparative proteomic analysis of Xanthomonas campestris pv. campestris 17.

Proteomics. 2007 Jun 13;7(12):2047-2058

Authors: Chung WJ, Shu HY, Lu CY, Wu CY, Tseng YH, Tsai SF, Lin CH

The bacterium Xanthomonas campestris pathovar campestris (XCC) 17 is a local isolate that causes crucifer black rot disease in Taiwan. In this study, its proteome was separated using 2-DE and the well-resolved proteins were excised, trypsin digested, and analyzed by MS. Over 400 protein spots were analyzed and 281 proteins were identified by searching the MS or MS/MS spectra against the proteome database of the closely related XCC ATCC 33913. Functional categorization of the identified proteins matched 141 (50%) proteins to 81 metabolic pathways in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. In addition, we performed a comparative proteome analysis of the pathogenic strain 17 and an avirulent strain 11A to reveal the virulence-related proteins. We detected 22 up-regulated proteins in strain 17 including the degrading enzymes EngXCA, HtrA, and PepA, which had been shown to have a role in pathogenesis in other bacteria, and an anti-host defense protein, Ohr. Thus, further functional studies of these up-regulated proteins with respect to their roles in XCC pathogenicity are suggested.

PMID: 17566974 [PubMed - as supplied by publisher]

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The crystallization of apo-form UMP kinase from Xanthomonas campestris is significantly improved in a strong magnetic field.

Acta Crystallograph Sect F Struct Biol Cryst Commun. 2007 May 1;63(Pt 5):438-42

Authors: Tu JL, Chin KH, Wang AH, Chou SH

Bacterial UMP kinases (UMPKs) are crucial enzymes that are responsible for microbial UTP biosynthesis. Interestingly, eukaryotic and prokaryotic cells use different enzymes for UMP-phosphorylation reactions. Prokaryotic UMPKs are thus believed to be potential targets for antimicrobial drug development. Here, the cloning, expression and crystallization of SeMet-substituted XC1936, a bacterial UMPK from Xanthomonas campestris pathovar campestris, are reported. The crystallization of the apo-form UMPK was found to be significantly improved in a strong magnetic field; the crystals diffracted to a resolution of 2.35 A, a dramatic improvement over the original value of 3.6 A. Preliminary structural analyses of apo-form XC1936 using crystals grown in a strong magnetic field clearly reveal well defined loop regions involved in substrate-analogue binding that were previously not visible. Crystallization in a strong magnetic field thus was found to be indispensable in determining the flexible region of the XC1936 UMPK structure.

PMID: 17565191 [PubMed - in process]

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Two type III effector genes of Xanthomonas oryzae pv. oryzae control the induction of the host genes OsTFIIA{gamma}1 and OsTFX1 during bacterial blight of rice.

Proc Natl Acad Sci U S A. 2007 Jun 11;

Authors: Sugio A, Yang B, Zhu T, White FF

Xanthomonas oryzae pv. oryzae strain PXO99(A) induces the expression of the host gene Os8N3, which results in increased host susceptibility to bacterial blight of rice. Here, we show that PXO99(A) affects the expression of two additional genes in a type III secretion system-dependent manner, one encoding a bZIP transcription factor (OsTFX1) and the other the small subunit of the transcription factor IIA located on chromosome 1 (OsTFIIAgamma1). Induction of OsTFX1 and OsTFIIAgamma1 depended on the type III effector genes pthXo6 and pthXo7, respectively, both encoding two previously undescribed members of the transcription activator-like (TAL) effector family. pthXo7 is strain-specific and may reflect adaptation to the resistance mediated by xa5, an allele of OsTFIIAgamma5 encoding a second form of the TFIIA small subunit on chromosome 5 of rice. The loss of pthXo6 resulted in reduced pathogen virulence, and ectopic expression of OsTFX1 abrogated the requirement for pthXo6 for full virulence. X. oryzae pv. oryzae therefore modulates the expression of multiple host genes using multiple TAL effectors from a single strain, and evidence supports the hypothesis that expression of the associated host genes contributes to host susceptibility to disease.

PMID: 17563377 [PubMed - as supplied by publisher]

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Regulation of bacterial RecA protein function.

Crit Rev Biochem Mol Biol. 2007 Jan-Feb;42(1):41-63

Authors: Cox MM

The RecA protein is a recombinase functioning in recombinational DNA repair in bacteria. RecA is regulated at many levels. The expression of the recA gene is regulated within the SOS response. The activity of the RecA protein itself is autoregulated by its own C-terminus. RecA is also regulated by the action of other proteins. To date, these include the RecF, RecO, RecR, DinI, RecX, RdgC, PsiB, and UvrD proteins. The SSB protein also indirectly affects RecA function by competing for ssDNA binding sites. The RecO and RecR, and possibly the RecF proteins, all facilitate RecA loading onto SSB-coated ssDNA. The RecX protein blocks RecA filament extension, and may have other effects on RecA activity. The DinI protein stabilizes RecA filaments. The RdgC protein binds to dsDNA and blocks RecA access to dsDNA. The PsiB protein, encoded by F plasmids, is uncharacterized, but may inhibit RecA in some manner. The UvrD helicase removes RecA filaments from RecA. All of these proteins function in a network that determines where and how RecA functions. Additional regulatory proteins may remain to be discovered. The elaborate regulatory pattern is likely to be reprised for RecA homologues in archaeans and eukaryotes.

PMID: 17364684 [PubMed - indexed for MEDLINE]

Scientist speaks on inputs, productivity role in agric development ...
Vanguard, Nigeria - Jun 14, 2007
Efforts by the Crop Crisis Control Project (C3P) to mitigate the effects of cassava mosaic virus disease and banana Xanthomonas wilt (BXW) in six countries ...

Scientist speaks on inputs, productivity role in agric development ...
Vanguard, Nigeria - Jun 14, 2007
Efforts by the Crop Crisis Control Project (C3P) to mitigate the effects of cassava mosaic virus disease and banana Xanthomonas wilt (BXW) in six countries ...

Scientist speaks on inputs, productivity role in agric development ...
Vanguard, Nigeria - Jun 14, 2007
Efforts by the Crop Crisis Control Project (C3P) to mitigate the effects of cassava mosaic virus disease and banana Xanthomonas wilt (BXW) in six countries ...

Scientist speaks on inputs, productivity role in agric development ...
Vanguard, Nigeria - Jun 14, 2007
Efforts by the Crop Crisis Control Project (C3P) to mitigate the effects of cassava mosaic virus disease and banana Xanthomonas wilt (BXW) in six countries ...

Scientist speaks on inputs, productivity role in agric development ...
Vanguard, Nigeria - Jun 14, 2007
Efforts by the Crop Crisis Control Project (C3P) to mitigate the effects of cassava mosaic virus disease and banana Xanthomonas wilt (BXW) in six countries ...

Scientist speaks on inputs, productivity role in agric development ...
Vanguard, Nigeria - Jun 14, 2007
Efforts by the Crop Crisis Control Project (C3P) to mitigate the effects of cassava mosaic virus disease and banana Xanthomonas wilt (BXW) in six countries ...

Scientist speaks on inputs, productivity role in agric development ...
Vanguard, Nigeria - Jun 14, 2007
Efforts by the Crop Crisis Control Project (C3P) to mitigate the effects of cassava mosaic virus disease and banana Xanthomonas wilt (BXW) in six countries ...

Scientist speaks on inputs, productivity role in agric development ...
Vanguard, Nigeria - Jun 14, 2007
Efforts by the Crop Crisis Control Project (C3P) to mitigate the effects of cassava mosaic virus disease and banana Xanthomonas wilt (BXW) in six countries ...

Scientist speaks on inputs, productivity role in agric development ...
Vanguard, Nigeria - Jun 14, 2007
Efforts by the Crop Crisis Control Project (C3P) to mitigate the effects of cassava mosaic virus disease and banana Xanthomonas wilt (BXW) in six countries ...

Scientist speaks on inputs, productivity role in agric development ...
Vanguard, Nigeria - Jun 14, 2007
Efforts by the Crop Crisis Control Project (C3P) to mitigate the effects of cassava mosaic virus disease and banana Xanthomonas wilt (BXW) in six countries ...

Scientist speaks on inputs, productivity role in agric development ...
Vanguard, Nigeria - Jun 14, 2007
Efforts by the Crop Crisis Control Project (C3P) to mitigate the effects of cassava mosaic virus disease and banana Xanthomonas wilt (BXW) in six countries ...

Scientist speaks on inputs, productivity role in agric development ...
Vanguard, Nigeria - Jun 14, 2007
Efforts by the Crop Crisis Control Project (C3P) to mitigate the effects of cassava mosaic virus disease and banana Xanthomonas wilt (BXW) in six countries ...

Scientist speaks on inputs, productivity role in agric development ...
Vanguard, Nigeria - Jun 14, 2007
Efforts by the Crop Crisis Control Project (C3P) to mitigate the effects of cassava mosaic virus disease and banana Xanthomonas wilt (BXW) in six countries ...

Scientist speaks on inputs, productivity role in agric development ...
Vanguard, Nigeria - Jun 14, 2007
Efforts by the Crop Crisis Control Project (C3P) to mitigate the effects of cassava mosaic virus disease and banana Xanthomonas wilt (BXW) in six countries ...

Scientist speaks on inputs, productivity role in agric development ...
Vanguard, Nigeria - Jun 14, 2007
Efforts by the Crop Crisis Control Project (C3P) to mitigate the effects of cassava mosaic virus disease and banana Xanthomonas wilt (BXW) in six countries ...

Strawberry growers must remain vigilant against angular leaf spot
Farmers Guardian, UK - Jun 13, 2007
DEFRA is reminding strawberry growers of the need to remain vigilant against the bacterial disease angular leaf spot (Xanthomonas fragariae), ...