Lymantria dispar iflavirus 1 (LdIV1), a new model to study iflaviral persistence in lepidopterans

Biblio

Publication Type:

Journal Article

Source:

J Gen Virol, Volume 95, Number Pt 10, p.2285-96 (2014)

ISBN:

1465-2099 (Electronic)<br/>0022-1317 (Linking)

Accession Number:

24986084

Keywords:

Animals, Cell Line, Genome, Viral, Larva/virology, Lepidoptera/ virology, Molecular Sequence Data, Polyproteins/genetics, RNA Viruses/ genetics/ isolation & purification/ultrastructure, RNA, Viral/ genetics, Sequence Analysis, DNA, Viral Proteins/genetics, Virion/ultrastructure

Abstract:

The cell line IPLB-LD-652Y, derived from the gypsy moth (Lymantria dispar L.), is routinely used to study interactions between viruses and insect hosts. Here we report the full genome sequence and biological characteristics of a small RNA virus, designated Lymantria dispar iflavirus 1 (LdIV1), that was discovered to persistently infect IPLB-LD-652Y. LdIV1 belongs to the genus Iflavirus. LdIV1 formed icosahedral particles of approx. 30 nm in diameter and contained a 10, 044 nt polyadenylated, positive-sense RNA genome encoding a predicted polyprotein of 2980 aa. LdIV1 was induced by a viral suppressor of RNA silencing, suggesting that acute infection is restricted by RNA interference (RNAi). We detected LdIV1 in all tested tissues of gypsy-moth larvae and adults, but the virus was absent from other L. dispar-derived cell lines. We confirmed LdIV1 infectivity in two of these cell lines (IPLB-LD-652 and IPLB-LdFB). Our results provide a novel system to explore persistent infections in lepidopterans and a new model for the study of iflaviruses, a rapidly expanding group of viruses, many of which covertly infect their hosts.

Notes:

Carrillo-Tripp, Jimena<br/>Krueger, Elizabeth N<br/>Harrison, Robert L<br/>Toth, Amy L<br/>Miller, W Allen<br/>Bonning, Bryony C<br/>Research Support, Non-U.S. Gov't<br/>Research Support, U.S. Gov't, Non-P.H.S.<br/>England<br/>J Gen Virol. 2014 Oct;95(Pt 10):2285-96. doi: 10.1099/vir.0.067710-0. Epub 2014 Jul 1.