An overlapping essential gene in the Potyviridae

Biblio

Publication Type:

Journal Article

Source:

Proc Natl Acad Sci USA, Volume 105, Number 15, p.5897-902 (2008)

ISBN:

1091-6490 (Electronic)<br/>0027-8424 (Linking)

Accession Number:

18408156

Keywords:

Genes, Essential, Genes, Overlapping, Genes, Viral, Genome, Viral, Open Reading Frames, Plant Viruses/ genetics, Polyproteins/genetics, Potyviridae/ genetics, RNA, Viral, Viral Fusion Proteins/ genetics

Abstract:

The family Potyviridae includes &gt;30% of known plant virus species, many of which are of great agricultural significance. These viruses have a positive sense RNA genome that is approximately 10 kb long and contains a single long ORF. The ORF is translated into a large polyprotein, which is cleaved into approximately 10 mature proteins. We report the discovery of a short ORF embedded within the P3 cistron of the polyprotein but translated in the +2 reading-frame. The ORF, termed pipo, is conserved and has a strong bioinformatic coding signature throughout the large and diverse Potyviridae family. Mutations that knock out expression of the PIPO protein in Turnip mosaic potyvirus but leave the polyprotein amino acid sequence unaltered are lethal to the virus. Immunoblotting with antisera raised against two nonoverlapping 14-aa antigens, derived from the PIPO amino acid sequence, reveals the expression of an approximately 25-kDa PIPO fusion product in planta. This is consistent with expression of PIPO as a P3-PIPO fusion product via ribosomal frameshifting or transcriptional slippage at a highly conserved G(1-2)A(6-7) motif at the 5' end of pipo. This discovery suggests that other short overlapping genes may remain hidden even in well studied virus genomes (as well as cellular organisms) and demonstrates the utility of the software package MLOGD as a tool for identifying such genes.

Notes:

Chung, Betty Y-W<br/>Miller, W Allen<br/>Atkins, John F<br/>Firth, Andrew E<br/>R01 GM067104/GM/NIGMS NIH HHS/United States<br/>R01 GM079523/GM/NIGMS NIH HHS/United States<br/>Research Support, N.I.H., Extramural<br/>Research Support, Non-U.S. Gov't<br/>United States<br/>Proc Natl Acad Sci U S A. 2008 Apr 15;105(15):5897-902. doi: 10.1073/pnas.0800468105. Epub 2008 Apr 11.