Translational frameshifting mediated by a viral sequence in plant cells


Publication Type:

Journal Article


Proc Natl Acad Sci USA, Volume 89, Number 6, p.2262-6 (1992)


0027-8424 (Print)<br/>0027-8424 (Linking)

Accession Number:



Amino Acid Sequence, Base Sequence, Cloning, Molecular, Codon, Frameshift Mutation, Glucuronidase/analysis/ genetics, Molecular Sequence Data, Oligodeoxyribonucleotides, Open Reading Frames, Plant Viruses/ genetics/isolation & purification, Plants/genetics/ microbiology, Plasmids, Polymerase Chain Reaction, Protein Biosynthesis, Protoplasts/physiology


<p>It has been proposed that the polymerase gene of barley yellow dwarf virus and related viruses is expressed by a ribosomal frameshift event during translation. The 5' end of this gene overlaps with the 3' end of an upstream gene that is in a different reading frame. The region of overlap is similar to sequences in retro- and coronaviruses that are known to express their polymerase genes by frameshifting. This overlap region includes a "shifty" heptanucleotide, followed by a highly structured region that may contain a pseudoknot. Sequences of 115 or 144 base pairs that span this region from barley yellow dwarf virus (PAV serotype) genomic RNA were introduced into a plasmid, so that a reporter gene could be expressed in plant cells only if a minus one (-1) frameshift event occurred. Frameshifting was detected at a rate of approximately 1%. This frameshifting was abolished when the stop codon at the 3' end of the upstream open reading frame was deleted. A sequence expected to form a strong stem-loop immediately upstream of the frameshift site was unnecessary for frameshifting, and initiation at AUG codons within the stem-loop appeared to be inhibited. Like viruses that infect hosts in other kingdoms, plant viruses also can induce frameshifting in translation of their genes.</p>


Brault, V<br/>Miller, W A<br/>Research Support, Non-U.S. Gov't<br/>Research Support, U.S. Gov't, Non-P.H.S.<br/>UNITED STATES<br/>Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2262-6.