In-vitro hatching experiments

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Construct microsieves from 18-mm and 20-mm test tube caps and 25 micrometer pore nylon mesh.
UV-sterilize all microsieves and hatch trays.

Arrange the hatching trays in replicated boxes using a pre-generated random order. Fill each hatching tray with 12 ml of the appropriate test or control solution.

Pipette between 5,000 and 10,000 surface-disinfested SCN eggs (depending on experimental details) onto each microsieve.

Incubate the experiment at room temperature in darkness. At every-other day intervals, transfer the microsieves to duplicate hatching trays containing fresh solution, and count the hatched SCN second-stage juveniles remaining in the original solution.

MicrosieveTransfering microsieves to fresh solution

Microsieves in crisper