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Staining and counting SCN eggs

After extracting the eggs from the cysts  in the prepared soil sample, place 4 to 5 drops of 1 M HCl and approximately one eye-dropper full of acid fuchsin stain into each beaker of egg solution. Heat beakers containing eggs and stain in the microwave until the suspension is about to boil (approximately 3 minutes for 12 samples). Remove beakers from microwave and allow to cool. Place beakers in the refrigerator until they are counted.