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In-vitro hatching experiments

Construct microsieves from 18-mm and 20-mm test tube caps and 25 micrometer pore nylon mesh.
UV-sterilize all microsieves and hatch trays.

Arrange the hatching trays in replicated boxes using a pre-generated random order. Fill each hatching tray with 12 ml of the appropriate test or control solution.

Pipette between 5,000 and 10,000 surface-disinfested SCN eggs (depending on experimental details) onto each microsieve.