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Surface Disinfestation of SCN Eggs
Extracting Nematodes from Soil with a Baermann Funnel
Protocol
Procedure:
- Assemble the Baermann funnel apparatus.
- A clamped rubber tube is placed below the funnel
- A piece of window screen (or similar material) is placed in the mouth of the funnel
- The funnel is placed into a rack or holder
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In-Vitro Hatching Studies
Purification of SCN Cysts by Sucrose Centrifugation
Staining Roots for Plant-Parasitic Nematode Visualization
Acid Fuchsin Stain Preparation
Protocol
Procedure:
- To 750 ml distilled H2O add 3.5 g Acid Fuchsin and 250 ml glacial acetic acid.
- Stir well.
Category:
Greenhouse Resistance Screening
Protocol
Soybean genotypes will be evaluated for resistance to soybean cyst nematode (SCN), Heterodera glycines, by growing the plants in infested soil-sand mix for approximately 30 days, after which the numbers of adult SCN females and cysts on the roots will be assessed. Subsequently, the reaction of individual plants of each soybean genotype to SCN will be classified based on the criteria presented by D.P. Schmitt and G. Shannon in "Differentiating Soybean Responses to Heterodera glycines races", 1992, Crop Science 32(1):275-277.
PROCEDURE:
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